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Freedom EVOlyzer®
Effective ELISA automation from Tecan

Laboratory managers worldwide are showing increasing interest in automation of ELISA processes. Tecan’s Freedom EVOlyzer offers a validated solution for automation of microplate­based chromogenic ELISAs, and can be easily adapted to meet the changing workflow and throughput requirements of today’s diagnostic laboratory.

The Freedom EVOlyzer is designed to reduce hands-on time for ELISA processing, helping to improve the workflow efficiency of busy diagnostic laboratories. Controlled by the easy to use Freedom EVOlution™ software, the platform offers outstanding flexibility for a wide variety of ELISA protocols. With scalable throughput, the Freedom EVOlyzer can grow with your laboratory needs, allowing simultaneous processing of four or more microplates in one run.

Key benefits of the Freedom EVOlyzer:

Ease of use – through seamless workflow integration
Walkaway processing – with dynamic scheduling and monitoring
Efficient throughput – matching today’s laboratory needs
Increased safety and reliability – with full sample traceability

The Freedom EVOlyzer has been developed to meet the requirements of Directive 98/79/EC-IVD and has been listed in the USA as a Class 1, 510(k) exempt medical device for diagnostic use.
Specifications:
Hardware features
Tip configuration EVOlyzer 100: 2 or 4 tips; EVOlyzer 150: 4 or 8 tips; EVOlyzer 200: 8 tips
Various combinations of different tip types are possible
Tip types Teflon®-coated stainless steel tips and disposable tips with or without filter (200/1000 μl)
Syringe size 1000 μl
Liquid handling features
Pipetting precision (CV) Volume Fixed tips Disposable tips 200 μl Disposable tips 1000 μl
Standard volumes * 10 μl ≤ 3.5% ≤ 3.5% -
  100 μl ≤ 0.75% ≤ 0.75% ≤ 1%
  * Typical results pipetting plasma in single pipetting mode (1000 μl syringe)
Liquid detection Down to 50 μl of conductive liquid can be detected in round-bottom microplates on standard
microplate carrier
Sample loading range Variable, starting with 16 sample tubes per grid up to 640 sample tubes (40 grids) depending on the worktable size
Sample input formats Sample tubes (tube diameter from approx. 10 up to 16 mm), microplates, strip plates or deep-well plates
Strip handling Up to 12 different assays per plate
Predilution options Common, serial or exclusive predilution, as well as several predilutions on one plate
Barcode identification Sample tubes, microplates, deep-well plates, strip plates, reagent containers
Supported formats Code 39, Code 128, Codabar 2 of 5 Interleaved
Barcode evaluation Reagent type, recording of lot number, lot expiry date
Incubation All incubators are suitable for dark incubation, and temperature is monitored
Ambient incubations Freedom EVOlyzer 100: 6 positions; Freedom EVOlyzer 150 & 200: 6/12 positions
Heated incubations Freedom EVOlyzer 100: 6 positions; Freedom EVOlyzer 150 & 200: 6/12 positions with or
without shaking
Temperature range RT-incubator: ambient temperature measured with accuracy of ± 1.5°C
  Heated incubator MIO-2™: from 5°C above ambient temperature up to 46 °C. Incubator is calibrated at 37°C or 46 °C with accuracy of +/- 0.5°C. The temperature accuracy over the plate +/- 1.0°C
Washer: HydroFlex™
Manifold 8-way manifold
Wash channels 4 channels for wash buffers, wash buffer capacities: 2 x 2 l and 2 x 4 l with liquid level sensing
Wash modes Strip or plate mode
Photometer: Sunrise™
Measurement range 340 - 3 99 nm: 0 - 3 .0 OD; 400 - 750 nm: 0 - 4 .0 OD
Wavelength selection 4 or 6 fixed interference filters (340 – 750 nm) or gradient filter (400 – 750 nm)
Measurement time Single wavelength: 6 seconds; dual wavelength: 8 seconds
Software Supporting 21 CFR part 11, operating system Windows® XP SP2
Interface Bidirectional ASTM* (NCCLS** LIS1*** /NCCLS LIS2) interface
Dimensions Freedom
EVOlyzer 100
Freedom
EVOlyzer 150
Freedom
EVOlyzer 200
Height 910 mm/35.8" 910 mm/35.8" 910 mm/35.8"
Width 1520 mm/59.8" 1890 mm/74.4" 2490 mm/98"
Depth 800 mm/31.5" 800 mm/31.5" 800 mm/31.5"
Weight 210 kg 260 kg 290 kg
Power supply requirements Power: 600 VA (EVOlyzer 100) and 1200 VA (EVOlyzer 150 & 200) Voltage:
100 - 120 VAC and 220 - 24 0 VAC, frequency 50/60 Hz
Operating conditions Temperature: 15 - 32 °C/59 - 90°F; relative humidity: 30 - 80% (non condensing) at 30°C/86°F
Compliance CE marked; compliant with the directive of in vitro diagnostic medical devices 98/79/EC
 
Tecan

Freedom EVOlyzer®

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