| Light source |
High energy xenon flash lamp |
| Wavelength range |
|
| Fluorescence Intensity top |
230 - 900 nm |
| Fluorescence Intensity bottom UV |
230 - 900 nm |
| Fluorescence Intensity bottom VIS |
380 - 900 nm |
| Fluorescence Polarization |
300 - 750 nm |
| Absorbance |
230 - 1000 nm |
| Detectors |
|
| Fluorescence |
Low dark current PMT (UV and red sensitive) |
| Absorbance |
UV silicon photodiode |
| Luminescence |
Photon-counting system with low dark current PMT |
| Plate formats |
6- to 1536-well plates |
| Temperature control |
4°C above ambient temperature up to 42°C |
| Shaking |
Linear, orbital, selectable amplitude |
| Fluorescence sensitivity values |
| Fluorescence top reading |
10 amol / well Fluorescein (Greiner® 384-well low volume black plate; 10 μl) |
| Fluorescence bottom reading |
0.4 fmol / well Fluorescein (Greiner 96-well SensoPlate™; 200 μl) |
| TRF |
0.5 amol / well Europium (Greiner 384-well low volume white plate; 10 μl, 100 flashes) |
| FP |
2 mP |
| Luminescence sensitivity value |
| Glow luminescence 384-well plate |
225 amol ATP / well (Greiner 384-well low volume white plate; 25 μl) |
| Flash luminescence 384-well plate |
12 amol ATP / well (Greiner 384-well white plate; 55 μl) |
| Absorbance |
| Accuracy (@ 492 nm, 0-2 OD) |
+/- 0.8% and 0.008 OD |
| Measurement range |
0 - 4 OD |
| Injectors |
| 3 different syringe sizes |
500 μl |
| |
1000 μl |
| |
2500 μl |
| Fastest Read Times (1 flash / well fluorescence intensity top) |
| 384 well plates |
Kinetic cycle |
< 19 sec |
| |
Plate in/plate out |
< 35 sec |
| 1536 well plates |
Kinetic cycle |
< 26 sec |
| |
Plate in/plate out |
< 40 sec |
