| Flexibility |
| Wavelength/Bandwidth/Accuracy (fluorescence intensity) |
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|
| Wavelength selection |
premium Quad4 Monochromators™ |
| Wavelength range |
Excitation: 230 nm - 850 nm; Emission: 280 nm - 850 nm |
| Bandwidth |
Adjustable ≤ 300 nm: 2.5 nm - 10 nm; > 300 nm: 5 nm - 20 nm |
| Wavelength accuracy |
≤ 300 nm: ± 0.5 nm; > 300 nm: ± 1 nm |
| Wavelength precision |
≤ 300 nm: ± 0.5 nm; > 300 nm: ± 1 nm |
| Wavelength/Bandwidth/Accuracy (absorbance) |
|
|
| Wavelength selection |
Double monochromator |
| Wavelength range |
230 nm - 1000 nm |
| Bandwidth |
2.5 nm below 300 nm, 5 nm above |
| Wavelength accuracy |
≤ 300 nm: ± 0.5 nm; > 300 nm: ± 1 nm |
| Wavelength precision |
≤ 300 nm: ± 0.5 nm; > 300 nm: ± 1 nm |
| Measurement range |
0 - 4 OD |
| Linearity |
0 - 3 OD: R2 ≥ 0.999 @ 280 nm and 492 nm |
| Accuracy at 492 nm |
1 OD: < ± 0.5 % and 0.006 OD |
| Precision at 492 nm |
1 OD: < ± 0.5 % and 0.006 OD |
| Sensitivity |
| Fluorescence sensitivity values |
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|
|
| Fluorescence top reading |
25 amol / well Fluorescein (Greiner® 384-well low volume black plate; 10 μl) |
| Fluorescence bottom reading |
0.6 fmol / well Fluorescein (Greiner 96-well SensoPate™; 200 μl) |
| TRF |
1.5 amol / well Europium (Greiner 384-well low volume white plate; 10 μl; 200 flashes) |
| FP 384-well plate |
2 mP standard deviation @1 nM Fluorescein |
| Luminescence sensitivity values |
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|
|
| Glow luminescence 384-well plate |
225 amol ATP / well (Greiner 384-well low volume white plate; 25 μl) |
| Flash luminescence 384-well plate |
12 amol ATP / well (Greiner 384-well white plate; 55 μl) |
| Speed |
Fastest read times -
fixed wavelength (on the fly) |
1536-well plate |
384-well plate |
96-well plate |
| Absorbance |
19 sec |
15 sec |
11 sec |
| Fluorescence top |
35 sec |
24 sec |
17 sec |
| Fluorescence bottom |
36 sec |
24 sec |
19 sec |
| FP |
100 sec |
42 sec |
22 sec |
| Fastest read times – scanning (450 nm – 550 nm, 5 nm step) |
96-well plate |
| Fluorescence top |
|
|
115 sec for spectra |
| Absorbance |
|
|
35 sec for spectra |
| Light sources |
High energy UV/VIS/NIR xenon flashlamp, light-emitting diodes |
| Detectors |
Fluorescence - extended wavelength (UV and red sensitive),
low dark current PMT |
| |
Luminescence - photon counting low dark current PMT;
96-, 384-, 1,536-well fibers |
| |
Absorbance - UV Silicon photodiode, 4 channel parallel reading |
| Miscellaneous |
|
| Plate formats |
6 - 1536-well plates |
| Temperature control |
Ambient + 4 °C to 42 °C |
| Shaking |
Linear, orbital, variable amplitude |
| Options |
| Injectors |
Pump speed 100 μl – 300 μl / sec ; syringe sizes 500 μl, 1,000 μl or 2,500 μl |
| Stacker |
Up to 50 microplates |
| Barcode |
Left or right |
