| Light Source |
UV Xenon flashlamp |
| Wavelength selection |
| Bandwidth |
Ex: < 5 nm for λ≤ 315 nm and < 9 nm for λ> 315 Em: < 20 nm |
Infinite M200 PRO
|
Quad-4 Monochromators system
(2 excitation and 2 emission monochromators) |
| |
Absorbance |
Fluorescence |
| Wavelength accuracy |
< ± 0,5 nm for λ> 315 nm
< ± 0.3 nm for λ≤ 315 nm |
< ± 2 nm for λ> 315 nm
< ± 1 nm for λ≤ 315 nm |
| Wavelength reproducibility |
< ± 0,5 nm for λ> 315 nm
< ± 0.3 nm for λ≤ 315 nm |
< ± 1 nm for λ> 315 nm
< ± 0.5 nm for λ≤ 315 nm |
Infinite F200 PRO
|
Up to 4 filter pairs per slide |
| Wavelength range |
| Fluorescence intensity |
Standard: Ex 230 - 600 nm, Em 330 - 600 nm
Optional: Ex 230 - 850 nm, Em 280 - 850 nm |
| Fluorescence polarization |
Standard: Ex 300 - 600 nm, Em 330 - 600 nm
Optional: Em 330 - 850 nm |
| Absorbance |
230 - 1000 nm |
| Detectors |
Fluorescence - PMT, optionally UV and red-sensitive
Absorbance - UV silicon photodiode
Luminescence - photon counting system with low dark current PMT |
| Plate formats |
6- to 384- well plates, cuvettes, NanoQuant Plate™ |
| Temperature control |
Ambient +5°C up to 42°C |
| Shaking |
Linear, orbital |
| Fluorescence sensitivity* values |
Infinite F200 PRO |
Infinite M200 PRO |
| Fluorescence Top reading* |
85 amol / well
(0,85 pM, 384-well plate) |
170 amol / well
(1,7 pM; 384-well plate) |
| FluorescenceI Bottom reading* |
0,7 fmol / well
(3,5 pM; 96-well plate) |
1,2 fmol / well
(6 pM; 96-well plate) |
| TRF** |
2,8 amol / well
(28 fM; 384-well plate) |
90 amol / well
(0,9 pM; 384-well plate) |
| FP* |
< 4 mP standard deviation
@ 1 nM Fluorescein |
N/A |
| Luminescence sensitivity values |
Standard |
|
| Glow luminescence*** |
225 amol ATP / well
(9 pM; low volume 384-well plate) |
|
| Flash luminescence**** |
12 amol ATP / well
(218 fM; 384-well plate) |
|
| Absorbance |
| Ratio accuracy 260 / 280 nm |
± 0,07 |
| Precision @ 260 nm |
< 0,2 % |
| Accuracy @ 260 nm |
< 0,5 % |
| Measurement range |
0 - 4 OD |
| Injectors |
| Pump speed |
100 - 300 µl/s |
| Injection volume |
selectable in 1 μl increments; max. volume: 800 μl per stroke |
| Dead volume |
100 µl including pump back |
| Fastest Read Times |
| 96 well plate |
20 sec |
| 384 well plate |
30 sec |
Wavelength Ex / Em-scan,
96 well plate 450 – 550 nm
5 nm step |
150 sec |
